To send a specimen, please print and complete the appropriate requisition form “tdap™ EGFR” or “Comprehensive” and fax to TMD at 630.789.3765.

Ship patient specimens to TMD at 610 Oakmont Lane Westmont, IL 60559. If a paraffin block is not available, 10 unstained slides (cut in 4-5 micron sections) from each site may be submitted.

TMD’s physician service team is available to assist you with specimen submission. We can be reached toll free at 866.897.7350.

Specimen Requirements for all IHC including EGFR pharmDx assay

Paraffin-embedded tumor tissues preserved in the following fixatives are suitable for all IHC testing including EGFR pharmDx™: 10% (v/v) neutral buffered formalin; 10% (v/v) unbuffered formalin; 25% (v/v) unbuffered formalin; AFA (acetic formalin alcohol); Richard-Allen Scientific’s Pen-fix; and Bouin’s fixative. Tissues fixed in Anatech’s PreFer are not suitable for EGFR pharmDx™ or other IHC assays. PreFer fixation results in unsatisfactory preservation of morphology for IHC analysis and may cause erroneous results.

Specimen shipping kits are also available for your convenience. Call 866.897.7350 or e-mail physicianservices@tmdlab.com to order tdap™ kits for your office.

Specimen Requirements for FISH
Paraffin-embedded tumor tissues preserved in the following fixatives are suitable for FISH testing: 10% (v/v) buffered formalin; Paraformaldehyde; Karnovsky’s fix (16%paraformaldehyde, 50% glutaraldehyde); Trump’s fix. Tissues fixed in the following fixatives are not compatible with FISH: Buin’s fix; Prefer fix; B5 fixative; Non-buffered formalin; Any acid-based fixatives (picric acid, etc); Mercuric chloride; Hollande’s fix; Michelle’s fix; Zamboni’s fix; Zenker’s fix; Formaldehyde/zinc fixative; Alcohols (any when used alone). Tissue samples should be optimally fixed in buffered formalin for no longer than 40 hours.